software
FreezeScan
Freezing behavior has found wide applications and has become a standard experiment in the study of learning and memory in areas such as molecular biology, genetics, and cognitive science. However, manual observation of freezing behavior is tedious, can contain subjective bias, and suffers from lack of reliability, consistency and practicality. Moreover, it is slow and labor intensive, thus making it impossible to conduct experiments in large quantities. FreezeScan is a tool for automatically detecting freezing states in rodents that fulfills this demand for high throughput screening in Fear Conditioning experiments.
-
Features.
-
Applications:
-
Complete Fear Conditioning Behavior Analysis System
-
Full turn‐key Hardware + Software System
-
Measures activity level for recognizing startle response
-
High‐Throughput capability available – typically 4 chambers but 8 or 9 possible
-
Real‐time or offline
-
Easy plug‐n‐play functionality
-
Pre‐programmable Interval Selection for various phases of a trial
-
Auto‐Interval Detection using cues in the video (LED on/off etc.), or from protocol
-
Multiple LED zones can be handled
-
Animal color modeling option for more accurate animal identification
-
No user intervention required during experiment
-
Detailed statistics about the freezing events that occurred during the experiment
-
Auto‐generated Interval Statistics, Binned Data Output, and Graphing and Charting included!
-
Validated to be more than 90% accurate with respect to human scoring
-
Automatic adaptation to changing environment, non‐uniform lighting, etc.
-
Result review, Visualization of Acquired Experiments
-
Extensive Experiment Database Management
-
Batch‐mode allows user to run multiple videos successively without human intervention
-
Contextual Fear Conditioning
-
Cued Fear Conditioning
-
Trace Fear Conditioning
-
Delay Fear Conditioning
-
Fear Extinction
-
Enpathy Test
Advanced Behavioral Insights
Clever Sys automates Fear Conditioning to eliminate scoring bias and inter-observer variability. Our validated technology enables researchers to quantify associative learning and memory with high-throughput efficiency and sub-second precision. By delivering instant, objective data, we provide the robust evidence necessary for preclinical regulatory compliance and the acceleration of CNS-related drug discovery and neurobehavioral phenotyping.
Tech Details
• Platform: Win 11/10
• Software: FreezeScan
• Hardware: Operant Chamber (or Empathy Chamber) with Accessories
Publications(partial)
-
Shi, S.M., Suh, R.J., Shon, D.J. et al. Glycocalyx dysregulation impairs blood–brain barrier in ageing and disease. Nature 639, 985–994 (2025). https://doi.org/10.1038/s41586-025-08589-9.
-
John V. Pluvinage, Michael S. Haney, Benjamin A. H. Smith, Jerry Sun, Tal Iram, Liana Bonanno, Lulin Li, Davis P. Lee, David W. Morgens, Andrew C. Yang, Steven R.Shuken, David Gate, Madeleine Scott, Purvesh Khatri, Jian Luo, Carolyn R. Bertozzi, Michael C. Bassik & Tony Wyss-Coray (2019) CD22 blockade restores homeostatic microglial phagocytosis in ageing brains, Nature volume 568, pages187–192 (2019).
-
Joseph M. Castellano, Kira I. Mosher, Rachelle J. Abbey, Alisha A. McBride, Michelle L. James, Daniela Berdnik, Jadon C. Shen, Bende Zou, Xinmin S. Xie, Martha Tingle, Izumi V. Hinkson, Martin S. Angst & Tony Wyss-Coray (2017) Human umbilical cord plasma proteins revitalize hippocampal function in aged mice, Nature (2017), doi:10.1038/nature22067.
-
.....
Optimized Windows Software
Proven Academic Trust
Our technology represents the industry benchmark for automated behavior testing. Scientific teams across the globe have published findings based on Clever Sys tech, adhering to strict preclinical compliance protocols while ensuring the highest level of data integrity and objectivity within prestigious international journals.
See high-fidelity AI-Powered Freezing Analysis in action: capturing exploration with sub-second precision to maintain rigorous preclinical standards.
Precision Analytics
High-resolution tracking engines delivering granular spatial data for complex behavioral phenotyping.
Instant Data Access
Dynamic processing enables immediate visualization and rapid validation of experimental trial flows.
Preclinical Compliance
Engineered to satisfy rigorous regulatory mandates for reproducible and valid preclinical research.
Seamless Integration
Modular architecture ensures interoperability with established lab infrastructure and data pipelines.