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Elevated-Plus & Elevated-Zero Maze: Advanced Anxiety-Like Behavior Analysis

Automated tracking of time and entries in open vs. closed segments for high-throughput behavioral exploration. Achieve precise metrics in rodent anxiety assays using state-of-the-art video analysis tech.

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behavior PARADIGM/test

Elevated-Plus / Elevated-Zero Maze Test

The Elevated-Plus Maze (EPM) and Elevated-Zero Maze (EZM) are critical tools for quantifying anxiety-related behavior by exploiting the conflict between exploration and fear of open spaces. While manual scoring is prone to human error and inconsistency, Clever Sys revolutionizes these assays with automated video-based behavior recognition. Our high-performance platform provides sub-second accuracy in detecting complex postures and zone transitions, significantly enhancing experimental speed and data reproducibility for preclinical research labs. By delivering objective, real-time analytics, Clever Sys helps scientists eliminate observer bias and optimize high-throughput drug discovery workflows. Our advanced instrumentation ensures your laboratory maintains the highest standards of preclinical compliance while producing robust, verifiable results across all behavioral trials.

  • Automated Analytics:  Advanced tracking algorithm removes subjective bias during elevated-plus and elevated-zero test.

  • Advanced Features:  Support Body-Across-Two-Area, Head-Dip and Stretch-Attend-Posture (SAP) Events besides general Arm Visit metrics,  

  • Validated Precision:       Used by top scientists to meet preclinical compliance and produce robust, verifiable research data.

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Traditional Methods

Manual Scoring

Observer Bias

Basic Insights

Time-Consuming 

Clever Sys AI

Fully Automated

Objective & Precise

Deep Data Analytics

Real-Time Analysis and Instant Results

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Advanced Behavioral Insights

Clever Sys automates EPM and EZM assays to eliminate scoring variability and human error. Our high-throughput platform captures objective behavioral data with sub-second precision, mapping pharmacological effects on anxiety and exploration. By delivering standardized, real-time analytics, we enable researchers to meet strict GxP preclinical compliance standards, accelerate CNS drug discovery pipelines, and ensure statistically robust reproducibility in every study.

Tech Details

• Platform:       Win 11/10

• Software:     TopScan

• Hardware:    Elevated-Plus Maze/Elevated-Zero Maze

Optimized Windows Software

Proven Academic Trust

Our technology represents the industry benchmark for automated behavior testing. Scientific teams across the globe have published findings based on Clever Sys tech, adhering to strict preclinical compliance protocols while ensuring the highest level of data integrity and objectivity within prestigious international journals.

See high-fidelity AI-Powered Elevated-Zero Maze Test Analysis in action: capturing exploration with sub-second precision to maintain rigorous preclinical standards.

Publications

  • Aron, L., Ngian, Z.K., Qiu, C. et al. Lithium deficiency and the onset of Alzheimer’s disease. Nature 645, 712–721 (2025). https://doi.org/10.1038/s41586-025-09335-x. 

  • Tian Z, Song J, Zhao X, Zhou Y, Chen X, Le Q, Wang F, Ma L, Liu X. The interhemispheric amygdala-accumbens circuit encodes negative valence in mice. Science. 2024 Nov 8;386(6722):eadp7520. doi: 10.1126/science.adp7520. Epub 2024 Nov 8. PMID:39509508.

  • Escamilla CO, Filonova I, Walker AK, Xuan ZX, Holehonnur R, Espinosa F, Liu S, Thyme SB, López-García IA, Mendoza DB, Usui N, Ellegood J, Eisch AJ, Konopka G, Lerch JP, Schier AF, Speed HE, Powell CM. Kctd13 deletion reduces synaptic transmission via increased RhoA. Nature. 2017 Nov 9;551(7679):227-231. doi: 10.1038/nature24470. Epub 2017 Nov 1. PMID: 29088697; PMCID: PMC5787033.

  • .....

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Precision Analytics

High-resolution tracking engines delivering granular spatial data for complex behavioral phenotyping.

Instant Data Access

Dynamic processing enables immediate visualization and rapid validation of experimental trial flows.

Preclinical Compliance

Engineered to satisfy rigorous regulatory mandates for reproducible and valid preclinical research.

Seamless Integration

Modular architecture ensures interoperability with established lab infrastructure and data pipelines.

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