
behavior PARADIGM/test
Hole-Board Test
The Hole-Board test is a widely used behavioral assay in behavioral neuroscience to evaluate exploratory behavior, anxiety-like responses, and neophilia in rodents. The apparatus consists of a flat board with evenly spaced holes, allowing measurement of head-dipping behavior as an index of curiosity and risk assessment. Increased head-dipping frequency and duration are typically interpreted as reduced anxiety and enhanced exploratory drive, while decreased activity suggests heightened anxiety or sedation.
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Clever Sys’s automated Hole-Board solution uses high‑resolution video tracking and AI-based behavior recognition to capture detailed paths, head-dipping and other related behaviors, . This fully automated workflow removes observer bias, increases throughput, and delivers reproducible, publication-ready data. Integrated analytics help preclinical teams rapidly quantify treatment effects and compare cohorts with confidence.
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Automated Analytics:Â Advanced tracking algorithm removes subjective bias during Hole-Board test.
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Advanced Features:Â Â Â Accurate Head-Dipping/Sniffing Event Detection.Â
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Validated Precision:Â Â Â Â Used by top scientists to meet preclinical compliance and produce robust, verifiable research data.



Traditional Methods


Manual Scoring

Observer Bias

Basic Insights
Time-ConsumingÂ
Clever Sys AI


Fully Automated

Objective & Precise

Deep Data Analytics
Real-Time Analysis and Instant Results

Advanced Behavioral Insights
Clever Sys automates Hole-Board assays by eliminating human variance and subjective scoring bias. Our advanced algorithms quantify the biological markers of neophilia and anxiety with superior temporal resolution. Rapid processing of complex spatial data provides the objective, reproducible evidence essential for GLP-compliant preclinical workflows and CNS drug discovery pipelines to ensure high-integrity research outcomes.
Tech Details
• Platform:    Win 11/10
• Software:   TopScan
• Hardware:  Hole-BoardApparatus

Optimized Windows Software
Proven Academic Trust
Our technology represents the industry benchmark for automated behavior testing. Scientific teams across the globe have published findings based on Clever Sys tech, adhering to strict preclinical compliance protocols while ensuring the highest level of data integrity and objectivity within prestigious international journals.
Publications
See high-fidelity AI-Powered Hole-Board Test Analysis in action: capturing exploration with sub-second precision to maintain rigorous preclinical standards.
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Aron, L., Ngian, Z.K., Qiu, C. et al. Lithium deficiency and the onset of Alzheimer’s disease. Nature 645, 712–721 (2025). https://doi.org/10.1038/s41586-025-09335-x.Â
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Tian Z, Song J, Zhao X, Zhou Y, Chen X, Le Q, Wang F, Ma L, Liu X. The interhemispheric amygdala-accumbens circuit encodes negative valence in mice. Science. 2024 Nov 8;386(6722):eadp7520. doi: 10.1126/science.adp7520. Epub 2024 Nov 8. PMID:39509508.
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Escamilla CO, Filonova I, Walker AK, Xuan ZX, Holehonnur R, Espinosa F, Liu S, Thyme SB, López-GarcÃa IA, Mendoza DB, Usui N, Ellegood J, Eisch AJ, Konopka G, Lerch JP, Schier AF, Speed HE, Powell CM. Kctd13 deletion reduces synaptic transmission via increased RhoA. Nature. 2017 Nov 9;551(7679):227-231. doi: 10.1038/nature24470. Epub 2017 Nov 1. PMID: 29088697; PMCID: PMC5787033.
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Precision Analytics
High-resolution tracking engines delivering granular spatial data for complex behavioral phenotyping.

Instant Data Access
Dynamic processing enables immediate visualization and rapid validation of experimental trial flows.

Preclinical Compliance
Engineered to satisfy rigorous regulatory mandates for reproducible and valid preclinical research.

Seamless Integration
Modular architecture ensures interoperability with established lab infrastructure and data pipelines.
