Automated Three-Chamber Sociability Test

Quantify social preference and social novelty in a single, standardized assay. Our fully automated, video-based Three-Chamber Sociability Test delivers objective, reproducible metrics for behavioral neuroscience, drug discovery, and preclinical safety studies—without manual scoring or observer bias.

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behavior PARADIGM/test

Three Chamber
Sociability Test

The Three-Chamber Sociability Test is a widely used paradigm for quantifying social preference and social novelty in rodents. Subjects freely explore three connected chambers, allowing researchers to measure time spent with a social partner versus a non-social stimulus, and later, a familiar versus a novel conspecific. Clever Sys fully automates this assay using advanced video-based tracking and behavior recognition. Our system objectively scores approach, investigation, and chamber occupancy without manual observation, dramatically reducing experimenter bias and labor. High-resolution data outputs, synchronized with experimental events, provide reproducible, publication-ready metrics that support behavioral neuroscience, drug discovery, and preclinical safety studies while meeting rigorous regulatory and compliance expectations.

Typical Behavior Events

Visit/Stay In Left Chamber
Visit/Stay In Right Chamber
Visit/Stay In Middle Chamber
Explore/Sniff Left Cylinder
Explore/Sniff Right Cylinder
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Validated Precision: Used by top scientists to meet preclinical compliance and produce robust, verifiable research data.

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Traditional Methods

Manual Scoring

Observer Bias

Basic Insights

Time-Consuming

Clever Sys AI

Fully Automated

Objective & Precise

Deep Data Analytics

Real-Time Analysis and Instant Results

Advanced Behavioral Insights

Clever Sys automates Social Partner Preference and Social Novelty testing in a single, streamlined Three-Chamber Sociability assay. Our AI-driven video analysis continuously tracks chamber occupancy, approach, and investigation behaviors, transforming raw video into high-resolution, quantitative metrics that are objective, reproducible, and ready for publication or regulatory review across diverse preclinical models.

Tech Details

• Platform: Win 11/10

• Software: SocialScan(TopScan with License for Corresponding Modules)

• Hardware: Three-Chamber Sociability Test Apparatus

Optimized Windows Software

Proven Academic Trust

Our technology represents the industry benchmark for automated behavior testing. Scientific teams across the globe have published findings based on Clever Sys tech, adhering to strict preclinical compliance protocols while ensuring the highest level of data integrity and objectivity within prestigious international journals.

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Publications

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See high-fidelity AI-Powered Three-Chamber Sociability Test Analysis in action: capturing exploration with sub-second precision to maintain rigorous preclinical standards.

Elizabeth A. Kleeman, Carolina Gubert, Sonali N. Reisinger, Kathryn C. Davidson, Da Lu, Merle Dayton, Liana Mackiewicz, Bethany A. Masson, Pranav Adithya, Alexandra L. Garnham, Gemma Stathatos, Moira K. O ’ Bryan, Rikeish R. Muralitharan, Francine Z. Marques, Shanshan Li, Huan Liao, Shae McLaughlin, Emmet T. Keough, Michelle Y. Wheeler, Pamudika Kiridena, Marcel Doerflinger, Marc Pellegrini, Anthony J. Hannan. Paternal SARSCoV-2 infection impacts sperm small noncoding RNAs and increases anxiety in offspring in a sex-dependent manner. Nature Communications, volume 16, Article number: 9045 (2025) .
https://www.nature.com/articles/s41467-025-64473-0#citeas..
Xu-Yi Li, Shi-Ya Zhang, Yi-Zhou Hong, Su Su Tang, tang_susu@126.com, Hao Wang, wanghao890625@126.com, Hao Hong, honghao@cpu.edu.cn. TGR5-mediated lateral hypothalamus-dCA3-dorsolateral septum circuit regulates depressive-like behavior in male
mice. Neuron, Volume 112, Issue 11p1795-1814.e10, June 05, 2024
https://www.cell.com/neuron/fulltext/S0896-6273(24)00152-1.
Christine Ochoa Escamilla, Irina Filonova, Angela K. Walker, Zhong X. Xuan, Roopashri Holehonnur, Felipe Espinosa, Shunan Liu, Summer B. Thyme, Isabel A. López-García, Dorian B. Mendoza, Noriyoshi Usui, Jacob Ellegood, Amelia J. Eisch, Genevieve Konopka, Jason P. Lerch, Alexander F. Schier, Haley E. Speed & Craig M. Powell (2017) Kctd13 deletion reduces synaptic transmission via increased RhoA. Nature. 2017 Nov 9;551(7679):227-231. doi: 10.1038/nature24470. Epub 2017 Nov 1. PMID: 29088697; PMCID: PMC5787033
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